TABLE 1.

Lymphocyte populations in mice

Tissue and cell typeNo. of cells (SD)a
Wild-type miceRhoG+/− miceRhoG−/− mice
Bone marrow
    Fractions A to Cb8.7 × 105 (2.7)8.4 × 105 (2.2)7.8 × 105 (1.7)
    Fraction D7.2 × 106 (1.5)7.2 × 106 (1.6)4.9 × 106 (0.8)
    Fraction E2.4 × 106 (0.5)2.6 × 106 (0.6)1.7 × 106 (0.3)
    Fraction F1.7 × 106 (0.3)2.0 × 106 (0.2)0.9 × 106 (0.2)
Thymus
    DP1.9 × 108 (0.6)ND2.4 × 108 (0.6)
    CD41.5 × 107 (0.4)ND1.8 × 107 (0.5)
    CD82.9 × 106 (1.1)ND3.2 × 106 (1.0)
    DN1.4 × 106 (0.4)ND1.5 × 106 (0.4)
Spleen
    CD4+1.7 × 107 (0.1)1.8 × 107 (0.1)2.0 × 107 (0.4)
    CD8+7.0 × 106 (1.7)7.0 × 106 (1.2)6.7 × 106 (1.6)
    MZ2.9 × 106 (1.0)ND2.4 × 106 (0.5)
    IgMhi IgDlo6.7 × 106 (1.3)ND7.4 × 106 (1.9)
    IgMhi IgDhi2.0 × 107 (1.1)ND1.3 × 107 (0.2)
    IgMlo IgDhi4.9 × 107 (1.1)ND4.1 × 107 (0.6)
Lymph node (%)
    CD4+39 (3)48 (2)42 (8)
    CD8+7 (2)7 (0)6 (1)
    B cells39 (8)26 (2)35 (11)
Peritoneum: IgM+CD5+ (%)26 (5)26 (8)26 (5)
  • a Values are the means (± SD) of flow cytometric analysis of 8-week-old mice. For determinantions of the number of cells in the bone marrow, T cells in the spleen, and cells in the lymph node and peritoneum corresponding to wild-type and heterozygous mutant mice, the number of mice (n) is 3. For RhoG homozygous mutant mice, n is 6; for determinations on the thymus, n is 10; and for B cell numbers in the spleen, n is 6. MZ, marginal zone B cells; ND, not done.

  • b Fractions were assessed using Hardy criteria (12).