TABLE 1.

S. cerevisiae strains used in this study

StrainGenotypeReference
MES111MATα his3-Δ200 leu2-3,112 ura3-52 trp1-Δ1 ade2 nme1-Δ2::TRP1 pMES127[CEN URA3 NME1]37
MES111-140MATα his3-Δ200 leu2-3,112 ura3-52 trp1-Δ1 ade2 nme1-Δ2::TRP1 pMES140[CEN LEU2 NME1]37
MES111-P6MATα his3-Δ200 leu2-3,112 ura3-52 trp1-Δ1 ade2 nme1-Δ2::TRP1 pMES140-P6[CEN LEU2 nme1-P6]37
TLG105-140MATα his3-Δ200 leu2-3,112 ura3-52 trp1-Δ1 ade2 nme1-Δ2::TRP1 xrn1-Δ1::KanMX4 pMES140[CEN LEU2 NME1]This study
TLG105-P6MATα his3-Δ200, leu2-3,112 ura3-52 trp1-Δ1 ade2 nme1-Δ2::TRP1 xrn1-Δ1::KanMX4 pMES140-P6[CEN LEU2 nme1-P6]This study
TLG136MATa ade2-1 his3-Δ200 leu2-3,112 trp1-Δ1 ura3-52 cdc15-1This study
YJA103MATα ade2-1 his3-Δ200 leu2-3,112 met15 trp1-Δ1 ura3-52 nme1-Δ2::TRP1 clb2Δ::KanMX4 pMES140 [LEU2, CEN, NME1]4
YSW1MATa POP4::TAPTAG::TRP1ks pep4::LEU2 nuc1::LEU2 sep1::URA3 trp1-1 his3-11,15 can-100 ura3-1 leu2-3,112This study