TABLE 1.

SCE analysis of BS cell transfectants containing the empty pcDNA3 vector (PSNG1 and PSNG13), pcDNA3-WT-BLM (PSNF5 and PSNP2), and pcDNA3-BLM-T99A/T122A (C20, C2.1, and C1.4)

Protein usedTransfectantNo. of chromosomes scoredNo. of SCEsNo. of SCEs/ chromosome
−BLM (vector only)PSNG19651,4611.51
PSNG134376721.54
+BLM (wild-type)PSNF56781880.28
PSNP21,0666830.64
+BLM (T99A/T122A)C205432450.44
C2.1544700.13
C1.49342780.3