TABLE 1.

S. cerevisiae strains used in this studya

StrainGenotypeSource or reference
W303-1A MAT a ade2 can1 his3 leu2 trp1 ura3 34
W303-1B MATα ade2 can1 his3 leu2 trp1 ura3 34
CTY10-5d MAT a ade2-101 his3200 leu21 trp1901 gal4 gal80 URA3::lexAop-lacZ Gift of R. Sternglanz
MCY4024CTY10-5d gal83Δ::TRP1 38
MCY4093 MATα gal83Δ::TRP1 ade2 can1 his3 leu2 trp1 ura3 H. Wiatrowski
MCY4094 MAT a sip1Δ::kanMX6 sip2Δ::kanMX4 ade2 can1 his3 leu2 trp1 ura3 H. Wiatrowski
MCY4095 MAT a sip1Δ::kanMX6 gal83Δ::TRP1 ade2 can1 his3 leu2 trp1 ura3 H. Wiatrowski
MCY4096 MAT a sip2Δ::kanMX4 gal83Δ::TRP1 ade2 can1 his3 leu2 trp1 ura3 H. Wiatrowski
MCY4099 MATα gal83Δ::TRP1 sip1Δ::kanMX6 sip2Δ::kanMX4 ade2 can1 his3 leu2 trp1 ura3 40
MCY4908W303-1A snf1Δ10 12
MCY4950 MAT a pak1Δ::kanMX4 sip2Δ::kanMX4 gal83Δ::TRP1 ade2 can1 his3 leu2 trp1 ura3 This study
MCY4951 MAT a pak1Δ::kanMX4 sip1Δ::kanMX6 sip2Δ::kanMX4 ade2 can1 his3 leu2 trp1 ura3 This study
MCY4952 MAT a pak1Δ::kanMX4 sip1Δ::kanMX6 gal83Δ::TRP1 ade2 can1 his3 leu2 trp1 ura3 This study
MCY4958 MAT a elm1Δ::URA3 sip1Δ::kanMX6 sip2Δ::kanMX4 ade2 can1 his3 leu2 trp1 ura3 This study
MCY4967 MATα pak1Δ::kanMX4 elm1Δ::URA3 sip2Δ::kanMX4 gal83Δ::TRP1 ade2 can1 his3 leu2 trp1 ura3 This study
MCY4969 MAT a tos3Δ::kanMX4 sip2Δ::kanMX4 gal83Δ::TRP1 ade2 can1 his3 leu2 trp1 ura3 This study
MCY4975 MATα tos3Δ::kanMX4 sip1Δ::kanMX6 gal83Δ::TRP1 ade2 can1 his3 leu2 trp1 ura3 This study
MCY4981 MAT a pak1Δ::kanMX4 gal83Δ::TRP1 ade2 can1 his3 leu2 trp1 ura3 This study
MCY4999W303-1A PAK1-GFP::kanMX6 This study
MCY5018 MAT a elm1Δ::URA3 sip2Δ::kanMX4 gal83Δ::TRP1 ade2 can1 his3 leu2 trp1 ura3 This study
MCY5025 MATα pak1Δ::kanMX4 snf1Δ10 ade2 can1 his3 leu2 trp1 ura3 This study
MCY5114W303-1A tos3Δ::kanMX4 13
MCY5115W303-1B pak1Δ::kanMX4 13
MCY5122 MAT a tos3Δ::kanMX4 elm1Δ::URA3 ade2 can1 his3 leu2 trp1 ura3 This study
MCY5123 MAT a pak1Δ::kanMX4 elm1Δ::URA3 tos3Δ::kanMX4 ade2 can1 his3 leu2 trp1 ura3 This study
MCY5125W303-1A elm1Δ::kanMX4 This study
MCY5134CTY10-5d pak1Δ::kanMX4 This study
MCY5137 MATα tos3Δ::kanMX4 sip1Δ::kanMX6 sip2Δ::kanMX4 ade2 can1 his3 leu2 trp1 ura3 This study
MCY5139 MAT a elm1Δ::URA3 sip1Δ::kanMX6 gal83Δ::TRP1 ade2 can1 his3 leu2 trp1 ura3 This study
MCY5140 MATα elm1Δ::URA3 sip1Δ::kanMX6 gal83Δ::TRP1 ade2 can1 his3 leu2 trp1 ura3 This study
  • a All strains have the W303 genetic background except MCY2649, MCY2693, and MCY2916, which have the S288C background, and CTY10-5d. Alleles are ade2-1, can1-100, his3-11,15, leu2-3,112, trp1-1, and ura3-1, except where otherwise noted.