TABLE 1.

S. cerevisiae strains used in this studya

StrainGenotypeSource or reference
W303-1AMATa ade2 can1 his3 leu2 trp1 ura334
W303-1BMATα ade2 can1 his3 leu2 trp1 ura334
CTY10-5dMATa ade2-101 his3200 leu21 trp1901 gal4 gal80 URA3::lexAop-lacZGift of R. Sternglanz
MCY4024CTY10-5d gal83Δ::TRP138
MCY4093MATα gal83Δ::TRP1 ade2 can1 his3 leu2 trp1 ura3H. Wiatrowski
MCY4094MATa sip1Δ::kanMX6 sip2Δ::kanMX4 ade2 can1 his3 leu2 trp1 ura3H. Wiatrowski
MCY4095MATa sip1Δ::kanMX6 gal83Δ::TRP1 ade2 can1 his3 leu2 trp1 ura3H. Wiatrowski
MCY4096MATa sip2Δ::kanMX4 gal83Δ::TRP1 ade2 can1 his3 leu2 trp1 ura3H. Wiatrowski
MCY4099MATα gal83Δ::TRP1 sip1Δ::kanMX6 sip2Δ::kanMX4 ade2 can1 his3 leu2 trp1 ura340
MCY4908W303-1A snf1Δ1012
MCY4950MATa pak1Δ::kanMX4 sip2Δ::kanMX4 gal83Δ::TRP1 ade2 can1 his3 leu2 trp1 ura3This study
MCY4951MATa pak1Δ::kanMX4 sip1Δ::kanMX6 sip2Δ::kanMX4 ade2 can1 his3 leu2 trp1 ura3This study
MCY4952MATa pak1Δ::kanMX4 sip1Δ::kanMX6 gal83Δ::TRP1 ade2 can1 his3 leu2 trp1 ura3This study
MCY4958MATa elm1Δ::URA3 sip1Δ::kanMX6 sip2Δ::kanMX4 ade2 can1 his3 leu2 trp1 ura3This study
MCY4967MATα pak1Δ::kanMX4 elm1Δ::URA3 sip2Δ::kanMX4 gal83Δ::TRP1 ade2 can1 his3 leu2 trp1 ura3This study
MCY4969MATa tos3Δ::kanMX4 sip2Δ::kanMX4 gal83Δ::TRP1 ade2 can1 his3 leu2 trp1 ura3This study
MCY4975MATα tos3Δ::kanMX4 sip1Δ::kanMX6 gal83Δ::TRP1 ade2 can1 his3 leu2 trp1 ura3This study
MCY4981MATa pak1Δ::kanMX4 gal83Δ::TRP1 ade2 can1 his3 leu2 trp1 ura3This study
MCY4999W303-1A PAK1-GFP::kanMX6This study
MCY5018MATa elm1Δ::URA3 sip2Δ::kanMX4 gal83Δ::TRP1 ade2 can1 his3 leu2 trp1 ura3This study
MCY5025MATα pak1Δ::kanMX4 snf1Δ10 ade2 can1 his3 leu2 trp1 ura3This study
MCY5114W303-1A tos3Δ::kanMX413
MCY5115W303-1B pak1Δ::kanMX413
MCY5122MATa tos3Δ::kanMX4 elm1Δ::URA3 ade2 can1 his3 leu2 trp1 ura3This study
MCY5123MATa pak1Δ::kanMX4 elm1Δ::URA3 tos3Δ::kanMX4 ade2 can1 his3 leu2 trp1 ura3This study
MCY5125W303-1A elm1Δ::kanMX4This study
MCY5134CTY10-5d pak1Δ::kanMX4This study
MCY5137MATα tos3Δ::kanMX4 sip1Δ::kanMX6 sip2Δ::kanMX4 ade2 can1 his3 leu2 trp1 ura3This study
MCY5139MATa elm1Δ::URA3 sip1Δ::kanMX6 gal83Δ::TRP1 ade2 can1 his3 leu2 trp1 ura3This study
MCY5140MATα elm1Δ::URA3 sip1Δ::kanMX6 gal83Δ::TRP1 ade2 can1 his3 leu2 trp1 ura3This study
  • a All strains have the W303 genetic background except MCY2649, MCY2693, and MCY2916, which have the S288C background, and CTY10-5d. Alleles are ade2-1, can1-100, his3-11,15, leu2-3,112, trp1-1, and ura3-1, except where otherwise noted.