TABLE 2.

Estimation of secondary structures in UBA peptides based on CD data

ProteinMethod of data analysis and averageb% of peptides with the following secondary structuresa:
HrHdBrBdTurnCoil
UBA (wild type)CDSSTR52.215.33.67.64.416.7
CONTIN/LL51.018.60.02.15.323.1
Average51.617.01.84.94.919.9
UBA1CDSSTR54.814.72.78.03.416.7
CONTIN/LL56.617.20.02.54.019.8
Average55.716.01.45.33.718.3
UBA2CDSSTR48.412.56.110.53.319.1
CONTIN/LL49.611.90.03.07.827.8
Average49.012.23.16.85.623.5
UBA3CDSSTR49.813.86.28.95.716.4
CONTIN/LL51.316.70.03.46.022.8
Average50.615.33.16.25.919.6
UBA4CDSSTR53.614.04.68.63.515.4
CONTIN/LL43.811.90.03.16.933.4
Average48.713.02.35.95.224.4
UBA5CDSSTR50.515.03.96.94.219.5
CONTIN/LL56.716.70.03.74.019.0
Average53.615.92.05.34.119.3
  • a Abbreviations: Hr, regular α-helix; Hd, distorted α-helix (number of amino acids equal to or less than 4); Br, regular β-strand; Bd, distorted β-strand (number of residues equal to or less than 2).

  • b The readings of CONTIN/LL are the averages of the results from the CONTIN and CONTINLL programs. Average, average of the results from CDSSTR and CONTIN/LL methods.